Chemi CL is a horseradish peroxidase substrate optimized for chemiluminescent Western blots imaged using X-ray film. Chemi CL requires as little as ten times less antibody than other substrates, allowing you to save precious antibodies and samples. Additionally, the Chemi CL signal is long lasting, allowing multiple exposures to be carried out without substantial signal decay.
Kit includes: Chemi CL Luminol/enhancer solution and Chemi CL Peroxide Chemiluminescent peroxide solution.
200ml, good for 25 mini blots.
Stronger signal than Amersham™ ECL or Pierce™ ECL
Detect low pg protein amounts
Use up to ten-times less antibody
Duplicate slot blots containing serial dilutions of transferrin were probed with the antibody dilutions shown, and detected with either Chemi CL or Pierce ECL (Thermo Scientific) according to the manufacturers’ instructions. The blots were imaged simultaneously on the same piece of film. Chemi CL produced the same signal with ten times less primary and five times less secondary antibody.