ArrayMix array hybridization instrument, 24-4 AM24-4
The ArrayMix can hold 1-4 microscope slides contained within 16-24 well sectioning chambers (chambers sold seperately) for a total of up to 96 arrays. Each slide makes direct contact with a thermal block to maintain accurate temperature control from 15ºC up to 90ºC, with a tolerance of 0.1ºC. Each of the three slides sits on an orbital shaker. The slides orbit around a small radius at an adjustable speed, from a low of 300 revolutions per minute to a maximum of 900 revolutions per minute. The orbital shaking allows for use of low concentration or dilute targets in hybridization, which saves money when using expensive peptides or antibodies for array experiments.
Mixing during hybridization can also be an essential step for success in array experiments. Mixing during hybridization can also be an essential step for success in array experiments, as referenced by the following publications:
Kinetics of antigen binding to antibody microspots: strong limitation by mass transport to the surface. Kusnezow W, Syagailo YV, Ruffer S, Klenin K, Sebald W, Hoheisel JD, Gauer C, Goychuk I. Proteomics. 2006 Feb;6(3):794-803.
Optimal design of microarray immunoassays to compensate for kinetic limitations: theory and experiment. Kusnezow W, Syagailo YV, Ruffer S, Baudenstiel N, Gauer C, Hoheisel JD, Wild D, Goychuk I. Mol Cell Proteomics. 2006 Sep;5(9):1681-96.
After hybridizations, the ArraySlide chambers can be removed and separated for scanning or further processing of the microarray slides. ArraySlide chambers sold separately.